Isolation and Identification of Phosphate Solubilizing Diazotrophic Endophytic Bacteria from the Root of Tomato Plant (Lycopersicon esculentum Mill. var. tymoti)

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Abdus Salam Junaedi, Tini Surtiningsih, Ni’matuzahroh    

ABSTRACT

This research aims to isolate endophytic bacteria from tomato roots (Lycopersicon esculentum Mill. var. Tymoti) that have ability to improve nitrogen element and solubilize phosphate elements. Data in this research analyzed descriptively by displaying visualization of test result. The result showed that there were eight isolates of endophytic bacteria that have ability to improve nitrogen element, those were: BEH1, BEH2, BEH3, BEH4, BEH5, BEH6, BEH7, and BEH8 isolate. There were four isolates from 8 isolates of diazotrophic endophytic bacteria that have ability to solubilize phosphate elements, those were BEH2, BEH4, BEH7, and BEH8 isolate. The diazotrophic endophytic bacteria species with phosphate solubilization ability were BEH2 (Enterobacter americana (90.07%)), BEH4 (Acinetobacter sp. (53.31%)), BEH7 (Bacillus firmus (77.27%)), and BEH 8 (Bacillus. cereus (68.18%)).

Key words:        Isolation, identification, diazotrophic endophytic bacteria, phosphate solubilization, bacteria, tomato plant (L. esculentum Mill. var. tymoti)

 

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